Tag Archives: intracellular

Intracellular (pipette) solution for whole-cell patch-clamp

This solution will be in dialysis with the cytosol and hence is similar to it in composition (high potassium and low sodium).

Chemical mM MW/concentration for 100 ml
KCl 107 74.55 0.7976 g
CaCl2 1 1 M 100 ul
MgCl2 7 1 M 700 ul
EGTA 11 380.35 0.4183
HEPES 10 238.3 0.2383 g
Na2ATP 5 569.16 0.2846

Adjust to pH to 7.2 using potassium hydroxide (KOH).

Always check the molecular weight on the bottles of the chemicals. It may vary, due to different water contents for example.


Chimerel et al., Bacterial metabolite indole modulates incretin secretion from intestinal enteroendocrine L cells, Cell Rep., 2014
Link to the article in PubMed