This solution will be in dialysis with the cytosol and hence is similar to it in composition (high potassium and low sodium).
| Chemical | mM | MW/concentration | for 100 ml |
| KCl | 107 | 74.55 | 0.7976 g |
| CaCl2 | 1 | 1 M | 100 ul |
| MgCl2 | 7 | 1 M | 700 ul |
| EGTA | 11 | 380.35 | 0.4183 |
| HEPES | 10 | 238.3 | 0.2383 g |
| Na2ATP | 5 | 569.16 | 0.2846 |
Adjust to pH to 7.2 using potassium hydroxide (KOH).
Always check the molecular weight on the bottles of the chemicals. It may vary, due to different water contents for example.
Reference
Chimerel et al., Bacterial metabolite indole modulates incretin secretion from intestinal enteroendocrine L cells, Cell Rep., 2014
Link to the article in PubMed